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High-Resolution Electron Microscopy$
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John C. H. Spence

Print publication date: 2013

Print ISBN-13: 9780199668632

Published to Oxford Scholarship Online: January 2014

DOI: 10.1093/acprof:oso/9780199668632.001.0001

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Imaging molecules: radiation damage

Imaging molecules: radiation damage

(p.154) 6 Imaging molecules: radiation damage
High-Resolution Electron Microscopy

John C. H. Spence

Oxford University Press

Chapter 6 is devoted to the imaging of radiation-sensitive materials, such as organic material, at high resolution using electron microscopy. The methods of single-particle imaging in cryo-electron microscopy and imaging two-dimensional crystals are described for structural biology, and additional sections review the theory of radiation damage and methods for its avoidance. The use of low voltages and tomography is also reviewed.

Keywords:   radiation damage, cryo-electron microscopy, tomography, single-particle imaging, TEM

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