Jump to ContentJump to Main Navigation
Crystals, X-rays and ProteinsComprehensive Protein Crystallography$
Users without a subscription are not able to see the full content.

Dennis Sherwood and Jon Cooper

Print publication date: 2010

Print ISBN-13: 9780199559046

Published to Oxford Scholarship Online: January 2011

DOI: 10.1093/acprof:oso/9780199559046.001.0001

Show Summary Details
Page of

PRINTED FROM OXFORD SCHOLARSHIP ONLINE (www.oxfordscholarship.com). (c) Copyright Oxford University Press, 2019. All Rights Reserved. An individual user may print out a PDF of a single chapter of a monograph in OSO for personal use. date: 05 December 2019

Complementary diffraction methods

Complementary diffraction methods

Chapter:
(p.595) 16 Complementary diffraction methods
Source:
Crystals, X-rays and Proteins
Author(s):

Dennis Sherwood

Jon Cooper

Publisher:
Oxford University Press
DOI:10.1093/acprof:oso/9780199559046.003.0016

Many of the biological functions of proteins depend on hydrogen atoms, or protons. Whilst the X-ray diffraction data obtained from most protein crystals do not allow the positions of hydrogen atoms to be determined experimentally, some crystals diffract to atomic resolution and these allow the electron density for individual hydrogen atoms to be seen. A more powerful means for defining hydrogen atom positions is to use neutrons, rather than X-rays, in a diffraction experiment, although this generally relies on the availability of large crystals. Neutrons are much more sensitive to hydrogen atoms than X-rays and the visibility of hydrogens can be further enhanced by replacing them with the isotope deuterium, either by soaking the crystals or by special expression methods (known as perdeuteration). This chapter discusses the essential physical properties of neutrons, the basis of neutron production and diffraction, and the practical aspects of data collection and analysis. It also describes X-ray methods for analysing short-lived reaction intermediates that rely on extremely rapid data collection (the Laue method).

Keywords:   atomic resolution, unrestrained refinement, neutron protein crystallography, deuterium, reactor, spallation source, time-of-flight analysis, data collection, perdeuteration, Laue diffraction

Oxford Scholarship Online requires a subscription or purchase to access the full text of books within the service. Public users can however freely search the site and view the abstracts and keywords for each book and chapter.

Please, subscribe or login to access full text content.

If you think you should have access to this title, please contact your librarian.

To troubleshoot, please check our FAQs , and if you can't find the answer there, please contact us .