Jump to ContentJump to Main Navigation
Crystals, X-rays and ProteinsComprehensive Protein Crystallography$
Users without a subscription are not able to see the full content.

Dennis Sherwood and Jon Cooper

Print publication date: 2010

Print ISBN-13: 9780199559046

Published to Oxford Scholarship Online: January 2011

DOI: 10.1093/acprof:oso/9780199559046.001.0001

Show Summary Details
Page of

PRINTED FROM OXFORD SCHOLARSHIP ONLINE (www.oxfordscholarship.com). (c) Copyright Oxford University Press, 2019. All Rights Reserved. An individual user may print out a PDF of a single chapter of a monograph in OSO for personal use. date: 12 December 2019

Experimental techniques: data collection and analysis

Experimental techniques: data collection and analysis

Chapter:
(p.362) 11 Experimental techniques: data collection and analysis
Source:
Crystals, X-rays and Proteins
Author(s):

Dennis Sherwood

Jon Cooper

Publisher:
Oxford University Press
DOI:10.1093/acprof:oso/9780199559046.003.0011

This chapter covers the essential methods by which X-rays are generated in the laboratory and at synchrotron sources for data collection from protein crystals. The methods by which X-rays of a suitable wavelength are selected and collimated for a diffraction experiment are described along with the underlying physical principles. The commonly used methods for protein data collection are then described with a summary of various area detector systems that are widely used in the field. The principles and practice of determining the X-ray diffraction intensities are then covered along with the physical basis of various correction factors which are applied to the data. The processes of scaling and merging, which allow a set of unique diffraction intensities to be obtained from the numerous redundant measurements made in a data collection, are described, along with methods for assessing the quality of the data. The effects which thermal motion and disorder within the crystal have on the diffraction intensities are discussed and appropriate correction factors are described along with a number of caveats, such as crystal twinning, which affect the subsequent steps of structure analysis.

Keywords:   X-ray sources, diffraction experiment, scaling, merging, rotation method, area detectors, data processing, auto-indexing, mosaic spread, intensity

Oxford Scholarship Online requires a subscription or purchase to access the full text of books within the service. Public users can however freely search the site and view the abstracts and keywords for each book and chapter.

Please, subscribe or login to access full text content.

If you think you should have access to this title, please contact your librarian.

To troubleshoot, please check our FAQs , and if you can't find the answer there, please contact us .