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Macromolecular Crystallization and Crystal Perfection$
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Naomi E. Chayen, John R. Helliwell, and Edward H. Snell

Print publication date: 2010

Print ISBN-13: 9780199213252

Published to Oxford Scholarship Online: May 2010

DOI: 10.1093/acprof:oso/9780199213252.001.0001

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Practical methods of crystallization

Practical methods of crystallization

Chapter:
(p.24) 3 Practical methods of crystallization
Source:
Macromolecular Crystallization and Crystal Perfection
Author(s):

Naomi E. Chayen

John R. Helliwell

Edward H. Snell

Publisher:
Oxford University Press
DOI:10.1093/acprof:oso/9780199213252.003.0003

There are four major techniques for growing crystals of biological macromolecules: vapour diffusion, microbatch, free interface diffusion and dialysis. Each method has its inherent advantages and disadvantages. The most popular techniques are vapour diffusion and microbatch, while the free interface diffusion has recently been gaining use in high‐throughput experiments. This chapter describes the methods and provides guidance to converting between the vapour diffusion and microbatch methods.

Keywords:   crystallization, vapour diffusion, free interface diffusion, dialysis, batch

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