Jump to ContentJump to Main Navigation
Neurovascular MedicinePursuing Cellular Longevity for Healthy Aging$
Users without a subscription are not able to see the full content.

Kenneth Maiese

Print publication date: 2009

Print ISBN-13: 9780195326697

Published to Oxford Scholarship Online: January 2010

DOI: 10.1093/acprof:oso/9780195326697.001.0001

Show Summary Details
Page of

PRINTED FROM OXFORD SCHOLARSHIP ONLINE (www.oxfordscholarship.com). (c) Copyright Oxford University Press, 2020. All Rights Reserved. An individual user may print out a PDF of a single chapter of a monograph in OSO for personal use. date: 24 February 2020

Glutamatergic Signaling In Neurogenesis

Glutamatergic Signaling In Neurogenesis

Chapter:
(p.269) Chapter 11 Glutamatergic Signaling In Neurogenesis
Source:
Neurovascular Medicine
Author(s):

Noritaka Nakamichi

Yukio Yoneda

Publisher:
Oxford University Press
DOI:10.1093/acprof:oso/9780195326697.003.0011

This chapter summarizes recent studies on the functional expression of ionotropic glutamate receptors (iGluRs) and metabotropic glutamate receptors (mGluRs) by neural progenitor cells isolated from embryonic rat and mouse brains. Cells are cultured in the presence of growth factors toward the formation of round spheres termed as neurospheres for different periods under floating conditions, whereas a reverse transcription polymerase chain reaction (RT-PCR) analysis reveals expression of messenger RNA for particular iGluR and mGluR subtypes in undifferentiated cells and neurospheres formed with clustered cells during the culture with growth factors. Sustained exposure to an agonist for the N-methyl-D-aspartate (NMDA) receptor not only inhibits the formation of neurospheres but also promotes spontaneous and induced differentiation of neurospheres into cells immunoreactive to a neuronal marker protein on immunocytochemistry and Western blot analyses. This chapter shows that glutamate could play a pivotal role in mechanisms underlying proliferation for self-renewal.

Keywords:   neural progenitor cells, neurospheres, NMDA receptors, metabotropic glutamate receptors, neurogenesis, ionotropic glutamate receptors, growth factors, glutamate, self-renewal

Oxford Scholarship Online requires a subscription or purchase to access the full text of books within the service. Public users can however freely search the site and view the abstracts and keywords for each book and chapter.

Please, subscribe or login to access full text content.

If you think you should have access to this title, please contact your librarian.

To troubleshoot, please check our FAQs , and if you can't find the answer there, please contact us .