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Measuring Metabolic RatesA Manual for Scientists$
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John R. B. Lighton

Print publication date: 2008

Print ISBN-13: 9780195310610

Published to Oxford Scholarship Online: September 2008

DOI: 10.1093/acprof:oso/9780195310610.001.0001

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Flow-Through Respirometry Using Excurrent Flow Measurement

Flow-Through Respirometry Using Excurrent Flow Measurement

Chapter:
(p.124) 11 Flow-Through Respirometry Using Excurrent Flow Measurement
Source:
Measuring Metabolic Rates
Author(s):

John R. B. Lighton

Publisher:
Oxford University Press
DOI:10.1093/acprof:oso/9780195310610.003.0011

This chapter describes the setup, the plumbing, and the equations for implementing a respirometry system wherein the flow rate of the air leaving the animal chamber is known. Such systems are usually referred to as pull systems, because the air is usually pulled from a chamber or mask at a known rate, and the concentrations of incurrent and excurrent gases are alternately measured. Such systems are often the only practical way of measuring the metabolic rates of large animals. Setups and equations for oxygen-only, carbon dioxide-only, and combined oxygen and carbon dioxide systems are described. Methods for creating multiple-animal pull-mode respirometry systems, for compensating flow rate, and for the automatic baselining (that is to say, measuring incurrent gas concentrations) of respirometry systems are discussed.

Keywords:   pull mode respirometry, respirometer mask, oxygen consumption, metabolic rate

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