Dealing with model uncertainty in reconstructing ancestral proteins in the laboratory: examples from archosaur visual pigments and coralfluorescent proteins
This chapter discusses efficient experimental strategies to explore variation in ancestral sequence reconstructions. The experimental strategies are illustrated with two examples, ancestral rhodopsins in archosaurs and green fluorescent protein (GFP)-like proteins in corals.
Keywords: ancestral sequence reconstruction, ancestral rhodopsins, archosaurs, green fluorescent protein, corals, gene synthesis
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