- Title Pages
- 1 Introduction
- 2 Crystallization theory
- 3 Practical methods of crystallization
- 4 Screening
- 5 Optimization
- 6 Strategies to apply when high‐quality crystals cannot be obtained
- 7 Membrane proteins
- 8 Alternative approaches
- 9 Experimental aspects
- 10 Analysis of the molecular short‐range order
- 11 Analysis of long‐range order
- 12 Macromolecular crystals and twinning
- 13 Other macromolecular crystal diffraction disorders
- 14 Degradation and improvement of crystal perfection
- 15 Unusual diffraction geometries
- 16 Making the most of difficult crystals — beamline and detector optimization
- 17 Protein powders — making the most of tiny crystallites in bulk
- 18 Complementary techniques
- 19 The X‐ray laser and the single molecule — no crystal needed?
- 20 Overall summary and future thoughts
- Glossary of abbreviations, terms and symbols
- (p.53) 7 Membrane proteins
- Macromolecular Crystallization and Crystal Perfection
Naomi E. Chayen
John R. Helliwell
Edward H. Snell
- Oxford University Press
The structural studies of membrane proteins lag those of their soluble counterparts. This is mainly due to the difficulty in crystallizing membrane proteins that have large hydrophobic regions that are in contact with the alkyl chains of the lipids in the membrane. This chapter describes specific methods for membrane protein crystal growth including screening with detergents, lipidic cubic‐phase crystallization and antibody fragment approaches.
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