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Macromolecular Crystallographyconventional and high-throughput methods$
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Mark R. Sanderson and Jane V. Skelly

Print publication date: 2007

Print ISBN-13: 9780198520979

Published to Oxford Scholarship Online: September 2007

DOI: 10.1093/acprof:oso/9780198520979.001.0001

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Phase refinement through density modification

Phase refinement through density modification

Chapter:
(p.143) CHAPTER 10 Phase refinement through density modification
Source:
Macromolecular Crystallography
Author(s):

Jan Pieter Abrahams

Jasper R. Plaisier

Steven Ness

Navraj S. Pannu

Publisher:
Oxford University Press
DOI:10.1093/acprof:oso/9780198520979.003.0010

This chapter discusses the concept of density modification, where a priori information about the structure of the macromolecule being studied is used to improve phase estimates. These concepts of density modification for the improvement of phases from crystallography have been implemented in many different programs in the past. Some of the popular density modification programs implementing the real space restraints are SOLOMON (Abrahams, 1997), DM (Cowtan, 1999), and RESOLVE (Terwilliger, 2003).

Keywords:   X-ray diffraction, phase measurement, electron density, density modification

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