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Quantitative Methods in Neuroscience
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Quantitative Methods in Neuroscience: A Neuroanatomical Approach

Stephen M. Evans, Ann Marie Janson, and Jens Randel Nyengaard

Abstract

Stereology is a valuable tool for neuroscientists, allowing them to obtain three-dimensional information from two-dimensional measurements made on appropriately sampled sections (usually obtained from histological sections or MRI/CT/PET scans). This 3-D information is invaluable in correlating structural/functional relationships in the pursuit of far greater understanding of the function of the central nervous system. However, in carrying out such measurements, often based on limited data sets, there is a risk of experimenter bias. An important feature of modern design based stereology is to b ... More

Keywords: stereology, disector, fractionator, sampling, length, surface area, volume, number, neuroscience

Bibliographic Information

Print publication date: 2004 Print ISBN-13: 9780198505280
Published to Oxford Scholarship Online: September 2009 DOI:10.1093/acprof:oso/9780198505280.001.0001

Authors

Affiliations are at time of print publication.

Stephen M. Evans, editor
Pharmaimaging Limited, Grangemouth, Scotland

Ann Marie Janson, editor
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden

Jens Randel Nyengaard, editor
Stereological Research Laboratory, Aarhus University Hospital, Aarhus, Denmark

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Contents

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Chapter 1 General introduction

Stephen M. Evans, and Jens R. Nyengaard

Chapter 2 A case study from neuroscience involving stereology and multivariate analysis

Luis M. Cruz-Orive, Ana M. Insausti, Ricardo Insausti, and Dámaso Crespo

Section 1 Number

Chapter 3 Section introduction

Stephen M. Evans, and Jens R. Nyengaard

Chapter 5 Counting in situ hybridized neurons

Bente Finsen, Rikke Gregersen, Elin Lehrmann, Steen Lovmand, and Mark J. West

Section 2 Volume

Chapter 8 Section introduction

Jens R. Nyengaard, and Stephen M. Evans

Section 3 Length and Surface

Chapter 11 Section introduction

Stephen M. Evans, and Jens R. Nyengaard

Section 4 Second Order Stereology

Section 5 Cell Culture

Chapter 15 Unbiased morphometrical techniques for the quantitative assessment of cells in primary dissociation cultures

H Schröder, J. R. Nyengaard, W Selberis, E Lain, A Keller, C Köhler, and B Witter

End Matter