This chapter describes the theory and practical applications of coulometric respirometry. Coulometric respirometry is probably the most accurate method for measuring oxygen consumption rates. It is ideal for small animals and has the dual advantages of high sensitivity and the fact that the oxygen in the organism's environment is not depleted, allowing measurements to continue for long periods in many cases. The technique works by maintaining a constant pressure in a sealed system by electrolytically producing oxygen at the same rate at which an enclosed organism consumes it.
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