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Measuring Metabolic Rates$
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John R. B. Lighton

Print publication date: 2008

Print ISBN-13: 9780195310610

Published to Oxford Scholarship Online: September 2008

DOI: 10.1093/acprof:oso/9780195310610.001.0001

Coulometric Respirometry

Chapter:
(p.18) 3 Coulometric Respirometry
Source:
Measuring Metabolic Rates
Author(s):

John R. B. Lighton

Publisher:
Oxford University Press
DOI:10.1093/acprof:oso/9780195310610.003.0003

This chapter describes the theory and practical applications of coulometric respirometry. Coulometric respirometry is probably the most accurate method for measuring oxygen consumption rates. It is ideal for small animals and has the dual advantages of high sensitivity and the fact that the oxygen in the organism's environment is not depleted, allowing measurements to continue for long periods in many cases. The technique works by maintaining a constant pressure in a sealed system by electrolytically producing oxygen at the same rate at which an enclosed organism consumes it.

Keywords:   oxygen consumption, constant pressure, small animals, high sensitivity

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