Flow-Through Respirometry Using Excurrent Flow Measurement
This chapter describes the setup, the plumbing, and the equations for implementing a respirometry system wherein the flow rate of the air leaving the animal chamber is known. Such systems are usually referred to as pull systems, because the air is usually pulled from a chamber or mask at a known rate, and the concentrations of incurrent and excurrent gases are alternately measured. Such systems are often the only practical way of measuring the metabolic rates of large animals. Setups and equations for oxygen-only, carbon dioxide-only, and combined oxygen and carbon dioxide systems are described. Methods for creating multiple-animal pull-mode respirometry systems, for compensating flow rate, and for the automatic baselining (that is to say, measuring incurrent gas concentrations) of respirometry systems are discussed.
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