Interpretation of Three-Dimensional Images of Macromolecules
This chapter covers all aspects of interpretation, starting with validation and consistency checks. Methods for visualization of the reconstruction by surface rendering are discussed, along with criteria for the definition of molecular boundaries. Segmentation is described both based on intrinsic properties of the reconstructed density distribution and on the use of tags such as gold labels or antibodies in the experiment. The chapter goes on to describe how a quasi-atomic model may be built by the fitting and docking of atomic coordinates into the 3D density map.
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