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Three-Dimensional Electron Microscopy of Macromolecular Assemblies
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Three-Dimensional Electron Microscopy of Macromolecular Assemblies: Visualization of Biological Molecules in Their Native State

Joachim Frank


In this book, the mathematical principles and working methods of single-particle reconstruction are described; a method designed to retrieve three-dimensional structural information from electron micrographs showing thousands of “copies” of biological molecules trapped in a thin layer of ice. This technique is uniquely suited to obtain three-dimensional images of molecular machines in different functional states, as it dispenses with the need for crystals. The book starts with an introduction of image formation in the electron microscope, which includes the definition of the contrast transfer ... More

Keywords: electron microscopy, image formation, projection angles, molecular machines, single-particle reconstruction, three-dimensional reconstruction

Bibliographic Information

Print publication date: 2006 Print ISBN-13: 9780195182187
Published to Oxford Scholarship Online: April 2010 DOI:10.1093/acprof:oso/9780195182187.001.0001


Affiliations are at time of print publication.

Joachim Frank, author
Howard Hughes Medical Institute