This chapter presents an autobiography of Shigetada Nakanishi. Nakanishi's early studies elucidated the characteristic precursor architectures of various neuropeptides and vasoactive peptides by introducing recombinant DNA technology. Subsequently, he established a novel functional cloning strategy for membrane receptors and ion channels by combining electrophysiology and Xenopus oocyte expression. He determined the molecular structure and elucidated the regulatory mechanisms of several peptide receptors as well as several G protein-coupled metabotropic-type and NMDA-type glutamate receptors. His early years, career, and achievements are discussed.
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